反轉錄酶III逆轉錄酶三invitrogen原裝18080-044

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反轉錄酶III 逆轉錄酶三 invitrogen原裝 SuperScript III Reverse Transcriptase

貨號18080-044

與SuperScriptII逆轉錄酶一樣，SuperScriptIII的RNase H 活性較低，能獲得更高產量的全長Cdna。另外，它還有著其它的逆轉錄酶所無法提供的熱穩定性；經過基因工程改造的SuperScriptIII具有更高的熱穩定性，其半衰期在50度時達到令人難以置信的220分鐘，這個特性也提高SuperScriptIII轉錄具有二級結構模板RNA的能力。

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invitrogen：www.invitrogen.com

產品描述

SuperScript III Reverse Transcriptase (RT) is a proprietary mutant of SuperScript II RT that is active at 50°C and has a half-life of 220 minutes, providing increased specificity with Gene-Specific Primers (GSPs) and the highest cDNA yield of all RTs. It is ideal for RT-PCR of a specific gene or generating cDNA from total or poly (A)⁺RNA sample. Like SuperScript II, it synthesizes a complementary DNA strand from single-stranded RNA, DNA, or an RNA:DNA hybrid. SuperScript III RT is genetically engineered by the introduction of point mutations that increase half-life, reduce RNase activity, and increase thermal stability.

Thermostability– Half life of 220 minutes at 50°C
Yield– Reduced RNase H activity for more full-length cDNA
Specificity– Full activity at 50°C for increased specificity with Gene Specific Primers
Applications– Array labeling, cDNA libraries, RT-PCR, primer extension, and 3 and 5 RACE

Source: Purified更看更多fromE. coliexpressing thepolgene of M-MLV (1, 2), mutagenized to increase thermal stability and half life and reduce RNase H activity.

Performance and Quality Testing: SDS-PAGE purity; endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays (Figure 2) and yield and length of cDNA product.

Unit Definition: One unit of SuperScript III RT is the amount of enzyme required to incorporate 1 nmole of deoxyribonucleotide into acid-precipitable material in 10 min. at 37°C using poly(A)漠氀椀zui漀(dT)_12-18as a template瀠爀椀洀攀爀 (3).

Unit Reaction Conditions: 50 mM Tris-HCl (pH 8.3), 40 mM KCl, 6 mM MgCl₂, 1 mM DTT, 0.5 mM [³H]dTTP, 0.1 mM poly(A), 0.1 mM oligo(dT)_12-18, 0.1 mg/ml BSA, and enzyme in 50 氀 for 10 min. at 37°C.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.